6KIJ
Crystal structure of yedK with ssDNA containing an abasic site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.444, 44.080, 55.148 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 23.170 - 1.580 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kbs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.901 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.640 |
| High resolution limit [Å] | 1.579 | 1.580 |
| Rmerge | 0.121 | 0.689 |
| Number of reflections | 30514 | 2980 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 6.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.2M Ammonium formate 20% w/v PEG3350 |
