6KIE

Crystal structure of human leucyl-tRNA synthetase, Leu-AMS-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	293
Detector technology	CCD
Collection date	2017-06-22
Detector	MAR CCD 165 mm
Wavelength(s)	1.00000
Spacegroup name	P 65 2 2
Unit cell lengths	138.774, 138.774, 448.843
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.848 - 3.150
R-factor	0.23
Rwork	0.229
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz2
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.110	0.056	0.423
Rmeas	0.119	0.060	0.495
Rpim	0.043	0.020	0.250
Total number of observations	280543
Number of reflections	46132	2472	2171
<I/σ(I)>	10.4
Completeness [%]	96.4	90.9	93
Redundancy	6.1	7.9	3.3
CC(1/2)		0.996	0.078

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24% PEG3350