6KI9

Apo structure of FabMG, novel types of Enoyl-acyl carrier protein reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	63.862, 137.005, 156.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.728 - 1.640
R-factor	0.217272860095
Rwork	0.217
R-free	0.24167
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.874
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.703
High resolution limit [Å]	1.640	1.644
Number of reflections	168488	16628
<I/σ(I)>	32.8
Completeness [%]	99.9
Redundancy	14
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M Bis-tris (pH 6.5), 23% (w/v) PEG 3350