6KI0
Crystal Structure of Human ASC-CARD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2011-07-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.760, 56.020, 146.930 |
| Unit cell angles | 90.00, 106.58, 90.00 |
Refinement procedure
| Resolution | 47.116 - 2.000 |
| R-factor | 0.2218 |
| Rwork | 0.220 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vd8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.994 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.082 | |
| Number of reflections | 92520 | 14772 |
| <I/σ(I)> | 12.21 | 1.76 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 3.75 | 3.78 |
| CC(1/2) | 0.998 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 1.80 M Ammonium Sulfate, 0.1 M HEPES 7.0 |
