6KHY

The crystal structure of AsfvAP:AG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	90
Detector technology	CCD
Collection date	2018-12-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	110.512, 148.504, 178.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.934 - 3.008
R-factor	0.2125
Rwork	0.211
R-free	0.24920
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.554
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.110
High resolution limit [Å]	3.000	6.440	3.000
Rmerge	0.085	0.043	0.362
Rmeas	0.091	0.045	0.405
Rpim	0.031	0.013	0.175
Total number of observations	215889
Number of reflections	28960	3070	2771
<I/σ(I)>	6.7
Completeness [%]	98.3	99.1	96
Redundancy	7.5	10.7	4.5
CC(1/2)		0.999	0.621

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	16% w/v PEG 3350 0.1 M Potassium sodium tartrate tetrahydrate