6KGB
Crystal structure of E61K mutated transthyretin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.538, 85.597, 64.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.799 - 1.300 |
| R-factor | 0.1617 |
| Rwork | 0.161 |
| R-free | 0.17670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pwe |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.716 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12-2829_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.822 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmeas | 0.029 | 0.849 |
| Rpim | 0.011 | 0.328 |
| Number of reflections | 58603 | 37155 |
| <I/σ(I)> | 29.5 | 2.5 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 27% PEG400, 0.2 M CaCl2, 0.1 M MES-NaOH pH 6.5 |
