6KDT

Crystal structure of human DNMT3B (Q772R)-DNMT3L complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2019-02-28
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	60.350, 240.784, 232.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.370 - 2.870
Rwork	0.194
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.127
Number of reflections	36776	2796
<I/σ(I)>	13.24
Completeness [%]	97.1
Redundancy	5.2
CC(1/2)		0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	2.2 M sodium formate