6KC6
HOIP-HOIPIN8 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.586, 88.793, 104.438 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 46.590 - 2.123 |
| R-factor | 0.2139 |
| Rwork | 0.211 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ljq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.117 | 1.783 |
| Number of reflections | 76335 | 12178 |
| <I/σ(I)> | 9.45 | 1.19 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 7.04 | 7.2 |
| CC(1/2) | 0.999 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 200 mM KCl, 20% PEG3,350 |
