6KC5
HOIP-HOIPIN1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.350, 60.227, 92.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.140 - 1.543 |
| R-factor | 0.1991 |
| Rwork | 0.198 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ljq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.445 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.062 | 1.580 |
| Number of reflections | 32942 | 5152 |
| <I/σ(I)> | 18.22 | |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 8.68 | 6.39 |
| CC(1/2) | 0.999 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES-Na (pH 7.5), 200 mM NaCl, 25% PEG 3,350 |
