6K9A
The complex of NrS-1 N terminal domain (1-305) with dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2018-05-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.114, 58.007, 47.135 |
| Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
| Resolution | 29.739 - 2.300 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.530 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.119 | 0.311 |
| Rmeas | 0.133 | 0.367 |
| Number of reflections | 14335 | 1390 |
| <I/σ(I)> | 8.7 | 2.4 |
| Completeness [%] | 98.4 | 95.7 |
| Redundancy | 4.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291.15 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4K |
