6K8N
Crystal structure of the Sulfolobus solfataricus topoisomerase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.671, 90.040, 156.184 |
| Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gaj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.885 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.942 |
| Number of reflections | 172718 | 8284 |
| <I/σ(I)> | 25.8 | 2 |
| Completeness [%] | 98.0 | 94.5 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 285 | 8% (v/v) Tacsimate (pH 4.4), and 13.75% (w/v) Polyethyleneglycerol 3,350 |
