6K68
Application of anti-helix antibodies in protein structure determination (8420-3MNZ)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2015-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.951, 95.017, 179.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.420 - 3.200 |
| R-factor | 0.2913 |
| Rwork | 0.289 |
| R-free | 0.31840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mnz 1dee |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.109 | 0.346 |
| Number of reflections | 22763 | 22763 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 20% PEG MME 2000, 0.1M MOPS pH 6.5 |
