6K65
Application of anti-helix antibodies in protein structure determination (9014-1P4B)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2016-09-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 35.620, 120.181, 84.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.217 - 1.650 |
R-factor | 0.1708 |
Rwork | 0.170 |
R-free | 0.19180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p4b 1dee |
RMSD bond length | 0.008 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rpim | 0.032 | 0.220 |
Number of reflections | 44481 | 4320 |
<I/σ(I)> | 31.8 | |
Completeness [%] | 99.5 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 1.8M NaK phosphate, 0.1M HEPES pH 7.5, |