6K5K
Structural and catalytic analysis of two diverse uridine phosphorylases in the oomycete Phytophthora capsici.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.790, 97.382, 188.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.691 - 2.097 |
| R-factor | 0.2497 |
| Rwork | 0.246 |
| R-free | 0.31700 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 72543 | 7120 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 283 | 0.1M sodium citrate tribasic dihydrate PH 5.0, 22% PEG 1000 |
