6K4Q
Crystal structure of xCas9 in complex with sgRNA and DNA (CGG PAM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2018-09-21 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.840, 69.669, 189.513 |
Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
Resolution | 49.380 - 2.700 |
R-factor | 0.20702 |
Rwork | 0.203 |
R-free | 0.27547 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4un3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.832 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.063 | 0.506 |
Number of reflections | 60255 | 5992 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.7 | 5.7 |
CC(1/2) | 0.995 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2M Ammonium phosphate dibasic, 20%(w/v) Polyethylene glycol 3,350 |