6K3M

Application of anti-helix antibodies in protein structure determination (8189-3LRH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-12
Detector	MAR CCD 130 mm
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	27.345, 56.102, 96.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.800
R-factor	0.19179
Rwork	0.190
R-free	0.23123
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lrh 1dee
RMSD bond length	0.011
RMSD bond angle	1.710
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rpim	0.022	0.037
Number of reflections	14114	14114
<I/σ(I)>	39.1
Completeness [%]	98.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	30% PEG 4000, 0.2M MgCl2, 0.1M MOPS pH 6.5