6K3E

LSD1/Co-Rest structure with an inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	121.510, 179.440, 234.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.460 - 2.870
R-factor	0.19028
Rwork	0.189
R-free	0.22282
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h6q
RMSD bond length	0.011
RMSD bond angle	1.909
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.460	76.340	2.940
High resolution limit [Å]	2.870	12.840	2.870
Rmerge	0.080	0.049	1.064
Number of reflections	58422	736	4288
<I/σ(I)>	13.5
Completeness [%]	99.4	98.9	99.7
Redundancy	6.8	6.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	36% Tacsimate