6K1T
The structure of Francisella virulence factor BioJ
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.943, 67.313, 55.571 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 40.208 - 1.584 |
| R-factor | 0.2082 |
| Rwork | 0.207 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.808 |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.320 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 37190 | 5609 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 91.5 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 289.15 | 20% (v/v) PEG 6000, 100mM BICINE/sodium hydroxide (pH 9.0) |
