6K13
Crystal Structure Basis for BmLDH Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97736 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 81.400, 88.204, 92.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.102 - 1.890 |
| R-factor | 0.1818 |
| Rwork | 0.180 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zvv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.772 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.329 | 1.923 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.025 |
| Number of reflections | 53697 | 53755 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium Chloride, 0.1 M Imidazole, HCl pH 8.0, 1.6 M K2HPO4 |
