6K0W
DNA methyltransferase in complex with sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 123.810, 88.480, 163.330 |
| Unit cell angles | 90.00, 96.50, 90.00 |
Refinement procedure
| Resolution | 50.503 - 2.650 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.25780 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.978 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.503 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.074 | 0.560 |
| Rpim | 0.045 | 0.339 |
| Number of reflections | 102152 | 14825 |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 20%PEG 5KMME,20%PEG 5KMME, 0.1M NaCl and 0.1M Tris pH 8.5 |
