6JZD
Crystal structure of peptide-bound VASH2-SVBP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.004, 111.339, 126.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.110 - 2.479 |
| R-factor | 0.1816 |
| Rwork | 0.179 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.943 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc3_3206: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.480 |
| Rmerge | 0.059 | 0.759 |
| Rpim | 0.025 | 0.332 |
| Number of reflections | 18801 | 2048 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 6.4 | 6.1 |
| CC(1/2) | 0.999 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | HEPES, potassium chloride, Ammonium sulphate |
