6JXQ

Room temperature structure of lysozyme delivered in polyacrylamide by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97942
Spacegroup name	P 43 21 2
Unit cell lengths	78.535, 78.535, 38.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.530 - 1.760
R-factor	0.1759
Rwork	0.174
R-free	0.20190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6irj
RMSD bond length	0.010
RMSD bond angle	1.033
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.300	1.820
High resolution limit [Å]	1.760	1.760
Number of reflections	17368	1211
<I/σ(I)>	7.5	1.6
Completeness [%]	100.0	100
Redundancy	770.4
CC(1/2)	0.994	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl