6JXQ
Room temperature structure of lysozyme delivered in polyacrylamide by serial millisecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 298.15 |
| Detector technology | PIXEL |
| Collection date | 2019-02-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.535, 78.535, 38.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.530 - 1.760 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6irj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.033 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.300 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 17368 | 1211 |
| <I/σ(I)> | 7.5 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 770.4 | |
| CC(1/2) | 0.994 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 293.5 | sodium acetate, PEG 8000, NaCl |
