6IRJ
Crystal structure of lysozyme by fixed-target serial femtosecond crystallography
Summary for 6IRJ
| Entry DOI | 10.2210/pdb6irj/pdb |
| Descriptor | Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | sfx, serial femtosecond crystallography, hydrolase |
| Biological source | Gallus gallus (Chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 14425.06 |
| Authors | Nam, K.H. (deposition date: 2018-11-13, release date: 2019-05-22, Last modification date: 2024-11-13) |
| Primary citation | Lee, D.,Baek, S.,Park, J.,Lee, K.,Kim, J.,Lee, S.J.,Chung, W.K.,Lee, J.L.,Cho, Y.,Nam, K.H. Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography. Sci Rep, 9:6971-6971, 2019 Cited by PubMed Abstract: Fixed-target serial femtosecond crystallography (FT-SFX) was an important advance in crystallography by dramatically reducing sample consumption, while maintaining the benefits of SFX for obtaining crystal structures at room temperature without radiation damage. Despite a number of advantages, preparation of a sample holder for the sample delivery in FT-SFX with the use of many crystals in a single mount at ambient temperature is challenging as it can be complicated and costly, and thus, development of an efficient sample holder is essential. In this study, we introduced a nylon mesh-based sample holder enclosed by a polyimide film. This sample holder can be rapidly manufactured using a commercially available nylon mesh with pores of a desired size at a low cost without challenging technology. Furthermore, this simple device is highly efficient in data acquisition. We performed FT-SFX using a nylon mesh-based sample holder and collected over 130,000 images on a single sample holder using a 30 Hz X-ray pulse for 1.2 h. We determined the crystal structures of lysozyme and glucose isomerase using the nylon mesh at 1.65 and 1.75 Å, respectively. The nylon mesh exposed to X-rays produced very low levels of background scattering at 3.75 and 4.30 Å, which are negligible for data analysis. Our method provides a simple and rapid but highly efficient way to deliver samples for FT-SFX. PubMed: 31061502DOI: 10.1038/s41598-019-43485-z PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.65 Å) |
Structure validation
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