6JXM
Crystal Structure of phi29 pRNA domain II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.606 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 120.178, 120.178, 187.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.180 - 3.320 |
| R-factor | 0.22702 |
| Rwork | 0.223 |
| R-free | 0.26515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mgm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.209 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.180 | 3.590 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.067 | |
| Rpim | 0.041 | 0.029 |
| Number of reflections | 10506 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.12 mM spermine, 5 mM cobalt hexamine, 10%(+/-)-2-methyl-2,4-pentanediol (MPD), 62 mM potassium chloride, 30 mM barium chloride, 40 mM pH 6 Na cacodylate |
