6JX5
Hect domain of AREL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 296 |
| Detector technology | PIXEL |
| Collection date | 2018-04-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 209.156, 121.057, 80.798 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 47.860 - 2.402 |
| R-factor | 0.1873 |
| Rwork | 0.186 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tug |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.627 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.860 | 2.488 |
| High resolution limit [Å] | 2.400 | 2.402 |
| Rpim | 0.049 | 0.235 |
| Number of reflections | 77372 | 7296 |
| <I/σ(I)> | 19.22 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.993 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 296.15 | 100mM Bis Tris (pH 5.5), 200mM Ammonium sulfate |
