6JX2

Crystal structure of Ketol-acid reductoisomerase from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-06
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	84.949, 90.179, 157.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.910 - 2.600
R-factor	0.1836
Rwork	0.179
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ypo
RMSD bond length	0.011
RMSD bond angle	1.548
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.910	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.091
Number of reflections	37218	37218
<I/σ(I)>	20.6
Completeness [%]	98.1
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25 % (w/v) polyethylene glycol (PEG) 3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M Magnesium Chloride