6JWC
Mouse antibody 2B5 Fab in complex with PEG
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-05 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.895, 98.895, 96.711 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.886 - 2.297 |
R-factor | 0.1889 |
Rwork | 0.187 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ju0 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.613 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.030 | 0.296 |
Number of reflections | 21952 | 2123 |
<I/σ(I)> | 25.3 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7 |
CC(1/2) | 0.954 | 0.808 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |