6JVX

Crystal structure of RBM38 in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	78.809, 78.809, 66.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.405 - 2.301
R-factor	0.1795
Rwork	0.177
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cqd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.101	0.058	0.942
Rmeas	0.113	0.065	1.057
Rpim	0.049	0.028	0.468
Total number of observations	53598
Number of reflections	10894	1151	1075
<I/σ(I)>	9.8
Completeness [%]	98.9	97.1	99.1
Redundancy	4.9	4.6	5
CC(1/2)		0.996	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.1M Tris-HCl (pH8.0), 2M ammonium sulfate