6JT9

Crystal Structure of D464A mutant of FGAM Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-12-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	146.461, 146.461, 141.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.520 - 2.100
R-factor	0.14753
Rwork	0.145
R-free	0.18891
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3t
RMSD bond length	0.019
RMSD bond angle	1.944
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rpim	0.048	0.185
Number of reflections	100187	4987
<I/σ(I)>	24.7
Completeness [%]	100.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2M Ammonium Sulphate