6JRP
Crystal structure of CIC-HMG-ETV5-DNA complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9778, 0.9785 |
| Spacegroup name | P 32 |
| Unit cell lengths | 66.082, 66.082, 158.285 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.791 - 3.000 |
| R-factor | 0.3006 |
| Rwork | 0.297 |
| R-free | 0.33430 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.109 | 0.704 |
| Number of reflections | 15474 | 727 |
| <I/σ(I)> | 26.56 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium acetate, PEG3350, Bis-tris pH 6.5 |
