6JOD

Angiotensin II type 2 receptor with ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-22
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	102.550, 426.430, 52.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.854 - 3.200
R-factor	0.2375
Rwork	0.235
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xjm 1m6t
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.854	3.310
High resolution limit [Å]	3.200	3.200
Rpim	0.050	0.551
Number of reflections	19767	1933
<I/σ(I)>	17.47
Completeness [%]	99.9
Redundancy	82.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	30% (v/v) PEG 300 0.1 M HEPES (pH 7.0) 100 mM Sodium phosphate dibasic dihydrate