6JO8
The complex structure of CHIKV envelope glycoprotein bound to human MXRA8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2018-07-11 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 208.790, 208.790, 299.739 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.150 - 3.495 |
| R-factor | 0.2495 |
| Rwork | 0.248 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n40 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.150 | 3.610 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rpim | 0.042 | 0.802 |
| Number of reflections | 95731 | 9457 |
| <I/σ(I)> | 21 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 130.4 | 127 |
| CC(1/2) | 0.999 | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.8M Ammomium phophate dibasic, 0.1M sodium acetate pH 6.0 |
