6JO0

Crystal structure of the DTS-motif rhodopsin from Phaeocystis globosa virus 12T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	45.194, 57.832, 117.037
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.160 - 1.651
R-factor	0.1926
Rwork	0.192
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b2n
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.190	45.190	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.086	0.046	1.628
Rmeas	0.094	0.051	1.767
Rpim	0.036	0.022	0.678
Number of reflections	37658	284	1819
<I/σ(I)>	10.2
Completeness [%]	99.9	98.9	99
Redundancy	6.6	5.5	6.6
CC(1/2)	0.998	0.996	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.5	293	Na-citrate (pH 5.5), 150 mM Na-phosphate, 25% PEG 600