6JMF

Crystal structure of human tyrosine-protein kinase Fes/Fps in complex with compound 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1
Spacegroup name	P 63
Unit cell lengths	80.790, 80.790, 131.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.19938
Rwork	0.197
R-free	0.24082
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bkb
RMSD bond length	0.008
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	32995
<I/σ(I)>	24.88
Completeness [%]	99.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1 M BisTrisPropane, 0.2 M Sodium Salfate, 15% PEG 3350, 10% Ethylene glycol