6JLY
eIF2a - eIF2B complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 155.563, 207.992, 155.595 |
Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
Resolution | 49.314 - 3.500 |
R-factor | 0.2245 |
Rwork | 0.224 |
R-free | 0.25980 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.587 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.710 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 123415 | |
<I/σ(I)> | 13 | |
Completeness [%] | 99.6 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium acetate, sodium citrate, PEG 4000, glycerol |