6JKI

Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	90
Detector technology	PIXEL
Collection date	2019-01-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.890575
Spacegroup name	P 32 2 1
Unit cell lengths	85.670, 85.670, 147.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.730 - 2.590
R-factor	0.209
Rwork	0.207
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gm8
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	2.730
High resolution limit [Å]	2.590	2.590
Rmeas	0.192
Number of reflections	37629	6010
<I/σ(I)>	14.38
Completeness [%]	99.8
Redundancy	19.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293.15	0.1M Tris-HCl, pH 8.4, 12.5% (v/v) MPD, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 5% (w/v) PEG 200