6JG0

Crystal structure of the N-terminal domain single mutant (S92E) of the human mitochondrial calcium uniporter fused with T4 lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	97.943, 97.943, 61.514
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.410 - 2.500
R-factor	0.1917
Rwork	0.188
R-free	0.25903
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lzm 4xtb
RMSD bond length	0.012
RMSD bond angle	1.402
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge		0.619
Rmeas		0.727
Rpim		0.368
Number of reflections	11124	586
<I/σ(I)>	14.3
Completeness [%]	99.7	99.8
Redundancy	4.8	3.5
CC(1/2)		0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20% PEG3350, 5% glycerol, 0.3 M (NH4)2SO4, and 0.1 M Bis-Tris-HCl (pH 5.5)