6JG0

Crystal structure of the N-terminal domain single mutant (S92E) of the human mitochondrial calcium uniporter fused with T4 lysozyme

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endolysin,Calcium uniporter protein	polymer	262	29830.2	1	UniProt (D9IEF7) UniProt (Q8NE86) Pfam (PF00959) In PDB	Enterobacteria phage T4 (Bacteriophage T4)	Lysis protein,Lysozyme,Muramidase
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3		water	water		18.0	77	Chemie (HOH)

Sequence modifications

A: 1 - 161 (UniProt: D9IEF7)

PDB	External Database	Details
Asn 20	Asp 20	engineered mutation
Thr 54	Cys 54	engineered mutation
Ala 97	Cys 97	engineered mutation
Gly 162	-	linker
Ser 163	-	linker

A: 1075 - 1165 (UniProt: Q8NE86)

PDB	External Database	Details
Glu 1092	Ser 92	engineered mutation
Leu 1166	-	expression tag
Glu 1167	-	expression tag
His 1168	-	expression tag
His 1169	-	expression tag
His 1170	-	expression tag
His 1171	-	expression tag
His 1172	-	expression tag
His 1173	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29830.2
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	30022.3

*Water molecules are not included.