6JDX

Crystal structure of AcrIIC2 dimer in complex with partial Nme1Cas9 preprocessed with protease alpha-Chymotrypsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97894
Spacegroup name	P 21 21 21
Unit cell lengths	55.374, 77.236, 107.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.003 - 2.280
R-factor	0.2087
Rwork	0.208
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jd7
RMSD bond length	0.019
RMSD bond angle	1.343
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.087	1.071
Rmeas	0.091	1.124
Rpim	0.027	0.335
Number of reflections	21775	1073
<I/σ(I)>	26.6	2.3
Completeness [%]	99.1	99.9
Redundancy	10.8	10.7
CC(1/2)	0.915	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.9	289	0.1M HEPES pH 7.9, 14% PEG 20000