6JDV
Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in catalytic state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.541, 158.340, 118.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.958 - 3.100 |
| R-factor | 0.2132 |
| Rwork | 0.212 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x2g |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.179 | 0.888 |
| Rmeas | 0.193 | 0.951 |
| Rpim | 0.070 | 0.336 |
| Number of reflections | 45911 | 2234 |
| <I/σ(I)> | 11.7 | 2.3 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.4 | 7.7 |
| CC(1/2) | 0.987 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 289 | Na citrate, 0.1M HEPES pH 8.0, 18.5% PEG 3350 |
