6JBF
Crystal structure of the bacterial ribosomal decoding site in complex with 4'-deoxy-4'-fluoro neamine analog (axial 4'-F)
Replaces: 5BWSExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.111, 81.324, 50.131 |
Unit cell angles | 90.00, 95.28, 90.00 |
Refinement procedure
Resolution | 42.543 - 2.600 |
R-factor | 0.1613 |
Rwork | 0.154 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.053 |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.543 | 42.543 | 2.660 |
High resolution limit [Å] | 2.600 | 11.610 | 2.600 |
Rmerge | 0.056 | 0.022 | 0.281 |
Rmeas | 0.061 | 0.024 | 0.305 |
Number of reflections | 6744 | 83 | 493 |
<I/σ(I)> | 25.15 | 52.12 | 7.63 |
Completeness [%] | 99.8 | 98.8 | 98.6 |
Redundancy | 6.782 | 6.169 | 6.85 |
CC(1/2) | 0.999 | 1.000 | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, NaCl, spermine tetrahydrochloride, sodium cacodylate |