6JAL
Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in ligand free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.040, 85.130, 55.340 |
| Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
| Resolution | 55.260 - 1.560 |
| R-factor | 0.1282 |
| Rwork | 0.127 |
| R-free | 0.15050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j9w |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.155 |
| Data reduction software | iMOSFLM (7.2.2) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.260 | 8.540 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.031 | 0.061 |
| Rmeas | 0.040 | 0.077 |
| Rpim | 0.024 | 0.048 |
| Number of reflections | 69683 | 3351 |
| <I/σ(I)> | 30.1 | 15 |
| Completeness [%] | 99.8 | 96.4 |
| Redundancy | 4.6 | 4.2 |
| CC(1/2) | 0.999 | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 277 | 0.05 M Citric Acid, 0.05 Bis-Tris Propane, 16% PEG 3350 |
