6J6X

Crystal structure of apo GGTaseIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.044
Spacegroup name	P 65 2 2
Unit cell lengths	88.540, 88.540, 647.581
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.475 - 2.962
R-factor	0.2674
Rwork	0.266
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dss 1dce
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.010
High resolution limit [Å]	2.960	2.960
Rmerge	0.204	1.848
Number of reflections	33185	1568
<I/σ(I)>	29
Completeness [%]	100.0	100
Redundancy	69.8	59.5
CC(1/2)		0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.04M MgCl2, 0.05M NaCacodylate pH 6, 5% MPD, 8mM CHAPS