6J64

Crystal structure of human HINT1 mutant complexing with AP4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	78.823, 46.414, 64.132
Unit cell angles	90.00, 94.23, 90.00

Refinement procedure

Resolution	32.436 - 0.950
R-factor	0.1658
Rwork	0.165
R-free	0.17540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eqe
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	0.970
High resolution limit [Å]	0.950	0.950
Rmerge	0.052	0.387
Number of reflections	137441	3690
<I/σ(I)>	30.8
Completeness [%]	95.0
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	HEPES pH 7.5, PEG3350