6J5S
Crystal structure of human HINT1 mutant complexing with AP5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.664, 46.475, 64.107 |
| Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
| Resolution | 32.421 - 1.020 |
| R-factor | 0.1623 |
| Rwork | 0.162 |
| R-free | 0.17510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eqe |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.040 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.040 | 0.456 |
| Number of reflections | 115657 | 3824 |
| <I/σ(I)> | 28.9 | |
| Completeness [%] | 98.7 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | HEPES pH 7.5, PEG 3350 |
