6J4V

Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	F 2 2 2
Unit cell lengths	82.281, 122.413, 194.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.045 - 2.100
R-factor	0.1704
Rwork	0.169
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j4o
RMSD bond length	0.007
RMSD bond angle	0.787
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.122	0.561
Rpim	0.040	0.196
Number of reflections	28792	2635
<I/σ(I)>	12.8	1.7
Completeness [%]	99.0	91.2
Redundancy	9.6	7.4
CC(1/2)		0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.2	293	For crystallization of V2c-SVBP C58S in complex with the maTail peptide the components were mixed in a 1:3 molar ratio and incubated at room temperature for 24 h before setting up crystallization trials. The resulting V2c-SVBP S58C-maTail complex was crystallized in 1.4 M sodium/potassium phosphate, pH 8.2.