6J4S
Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.198, 111.523, 127.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.304 - 2.800 |
| R-factor | 0.1749 |
| Rwork | 0.172 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j4o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.147 | 1.203 |
| Rpim | 0.048 | 0.505 |
| Number of reflections | 14701 | 1410 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 10.3 | 6.3 |
| CC(1/2) | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.8 | 293 | full-length V2-SVBP complex at a concentration of 15 mg/mL was crystallized in 1.8 M sodium/potassium phosphate, pH 6.8 |
