6J4P

Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 32 2 1
Unit cell lengths	63.724, 63.724, 151.256
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.223 - 1.599
R-factor	0.1655
Rwork	0.164
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j4o
RMSD bond length	0.006
RMSD bond angle	0.970
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.660
High resolution limit [Å]	1.599	1.600
Rmerge	0.077	0.228
Rpim	0.026	0.075
Number of reflections	48003	4722
<I/σ(I)>	26.8
Completeness [%]	100.0	100
Redundancy	9.9	10.2
CC(1/2)	0.995	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	V2c-SVBP was mixed with the inhibitor epoY at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-epoY was crystallized in 1.0 M lithium chloride, 0.1 M HEPES, pH 7.0, 20% PEG 6000.