6J44
Crystal structure of the redefined DNA-binding domain of human XPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.28240 |
| Spacegroup name | P 3 |
| Unit cell lengths | 66.982, 66.982, 35.462 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.460 - 2.060 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.23920 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.125 | 0.442 |
| Rmeas | 0.132 | 0.468 |
| Rpim | 0.041 | 0.154 |
| Number of reflections | 10924 | 1077 |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.8 M Ammonium citrate tribasic |
